Homogeneous assay for evaluation of aptamer-protein interaction.
نویسندگان
چکیده
We introduce Amplified Luminescent Proximity Homogenous Assay (ALPHA) to assess the K(D) value of aptamer-protein complexes as demonstrated through the study of apple stem pitting virus coat protein-specific aptamers. This method can be used as a simple, cost-effective method for screening aptamer-target protein interactions during aptamer selection.
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ورودعنوان ژورنال:
- The Analyst
دوره 137 17 شماره
صفحات -
تاریخ انتشار 2012